Preparation and Administration of 4TM Solution

4TM (H6278, Sigma-Aldrich Chemie N.V., Netherlands) was prepared as described previously (21 (link)). First, 10 mg of 4TM was first dissolved in 200 μl of 100% dimethyl sulfoxide (DMSO) (D8418, Sigma-Aldrich Chemie N.V., Netherlands). This stock solution was stepwise-diluted in 1900 μl of saline containing 2%Tween 80 (P1754, Sigma-Aldrich Chemie N.V., Netherlands) and then once more in the same volume of saline. This resulted in a final solution of 4TM (2.5 mg/ml), 5% DMSO, and 1% Tween 80 in saline. Animals received 4TM [25 mg/kg, intraperitoneally (ip)] 2 hours after a tag session (see below).

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Visser E., Matos M.R., van der Loo R.J., Marchant N.J., de Vries T.J., Smit A.B, & van den Oever M.C. (2020). A persistent alcohol cue memory trace drives relapse to alcohol seeking after prolonged abstinence. Science Advances, 6(19), eaax7060.

Publication 2020

H6278 D8418 P1754

Animals Dimethyl sulfoxide Saline Tween 80

Corresponding Organization : Vrije Universiteit Amsterdam

Other organizations : Amsterdam UMC Location VUmc

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Variable analysis

independent variables

Dose of 4TM (25 mg/kg, intraperitoneally (ip))

dependent variables

Not explicitly mentioned

control variables

Concentration of 4TM (2.5 mg/ml)
Concentration of DMSO (5%)
Concentration of Tween 80 (1%)
Vehicle (saline)

positive controls

Not specified

negative controls

Not specified

Annotations

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