Enterocyte-like HT29 cells (39 (link)) were used for analysis with the MUSE Cell Analyzer (Merck KGaA, Darmstadt, Germany). HT29 cells were obtained from American Type Culture Collection (Manassas, VA, USA). Cells were routinely grown in Dulbecco's modified Eagle's minimal essential medium (DMEM) (Nacalai Tesque, Inc., Kyoto, Japan) supplemented with 10% sterilized fetal bovine serum (Valley Biomedical, Inc., Winchester, VA, USA) and 1% antibiotic/antimycotic mixed stock solution (Nacalai Tesque, Inc.). HT29 cells were incubated in 5% CO2 at 37˚C. Cell treatment was performed as previously described (40 (link)).
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HT29 Cell Culture and Analysis
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Corresponding Organization :
Other organizations : Tohoku University, Sapporo Medical University
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Variable analysis
independent variables
- Cell treatment
- Cell analysis with MUSE Cell Analyzer
- Cell type (HT29 cells)
- Cell culture medium (DMEM supplemented with 10% fetal bovine serum and 1% antibiotic/antimycotic)
- Cell incubation conditions (5% CO2 at 37°C)
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