Protocol detail

Culturing wt-SiHa and IFITM Null Cells

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The wt-SiHa cells and IFITM1/IFITM3 null cells, which were described previously [43 (link)], were grown in RPMI 1640 medium (Invitrogen, Waltham, MA, USA), supplemented with 10% (v/v) fetal bovine serum (Labtech, East Sussex, UK) and 1% penicillin/streptomycin (Invitrogen, Waltham, MA, USA), and incubated at 37 °C with 5% CO₂.

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Gómez-Herranz M., Faktor J., Yébenes Mayordomo M., Pilch M., Nekulova M., Hernychova L., Ball K.L., Vojtesek B., Hupp T.R, & Kote S. (2022). Emergent Role of IFITM1/3 towards Splicing Factor (SRSF1) and Antigen-Presenting Molecule (HLA-B) in Cervical Cancer. Biomolecules, 12(8), 1090.

Publication 2022

RPMI 1640 medium fetal bovine serum penicillin/streptomycin

Cells Fetal bovine serum Ifitm1 Null cells Penicillin Streptomycin

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Variable analysis

independent variables

Type of cells: wt-SiHa cells and IFITM1/IFITM3 null cells

dependent variables

Not explicitly mentioned

control variables

RPMI 1640 medium (Invitrogen, Waltham, MA, USA)
10% (v/v) fetal bovine serum (Labtech, East Sussex, UK)
1% penicillin/streptomycin (Invitrogen, Waltham, MA, USA)
Incubation at 37 °C with 5% CO2

controls

The wt-SiHa cells and IFITM1/IFITM3 null cells were used as experimental groups, as described in the referenced publication [43].

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