Four TSNA were analyzed: NNN, NNK, N′-nitrosoanatabine (NAT) and N′-nitrosoanabasine (NAB). Briefly, [13C6]NNN and [pyridine-D4]NNK internal standards were applied directly to the filter pads which were then extracted in 15 ml 10 mM ammonium acetate buffer by sonication for one hour. The extracts were then purified on ChemElut (Varian, Harbor City, CA) and Sep-Pak Plus silica cartridges (Waters, Milford, MA). The purified samples were analyzed by LC-MS/MS in selected reaction monitoring mode as previously described.25 (link)
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Quantification of Tobacco-Specific Nitrosamines
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Other organizations : Georgetown University, University of Minnesota
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Variable analysis
independent variables
- None explicitly mentioned
- Levels of four TSNA: NNN, NNK, N'-nitrosoanatabine (NAT) and N'-nitrosoanabasine (NAB)
- Use of internal standards [13C6]NNN and [pyridine-D4]NNK
- Extraction in 15 ml 10 mM ammonium acetate buffer by sonication for one hour
- Purification on ChemElut and Sep-Pak Plus silica cartridges
- Analysis by LC-MS/MS in selected reaction monitoring mode
- Positive control: Not mentioned
- Negative control: Not mentioned
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