KatushkaS158A (tfRFP) were purified as described 28 (link). Briefly, E. coli BL21 (DE3) cells were transformed with pRSET-tfRFP. After full expression, the collected bacteria were frozen at -80 ℃ for at least 30 mins, then thawed and broken by ultrasound. tfRFP was purified by His-Tag Purification Resin (Roche, Basel, Switzerland). The obtained protein was dialyzed in PBS and diluted to a concentration of 2 mg/mL.
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