Biofilm Metal Resistance Evaluation

To assess the metal resistance of the produced biofilms, Pb²⁺, Zn²⁺, Cd²⁺, Cu²⁺, and Ni²⁺ salts were generated from Pb(NO₃)₂, ZnSO₄, CdSO₄, CuSO₄, and NiSO₄ salts (Sigma-Aldrich, St. Louis, MO, USA). No more than 60 min before usage, working solutions were produced in TSB medium from stock solutions. Based on the previous research (Basak et al., 2014 (link)) and the preliminary test, a concentration range of 100,000–781 μg/ml was chosen (Buzejić et al., 2016 (link)).

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Jakovljević V., Grujić S., Simić Z., Ostojić A, & Radojević I. (2022). Finding the best combination of autochthonous microorganisms with the most effective biosorption ability for heavy metals removal from wastewater. Frontiers in Microbiology, 13, 1017372.

Publication 2022

Pb(NO3)2 ZnSO4 CdSO4 CuSO4 NiSO4

Biofilms Metal Salts

Corresponding Organization : University of Kragujevac

Other organizations : State University of Novi Pazar

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Variable analysis

dependent variables

Metal resistance of the produced biofilms

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Concentration range of 100,000–781 μg/ml

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