Xenograft Experiments in MM Model

Xenograft experiments were performed as previously described.²⁹ (link) In summary, 1.0 × 10⁷ MM cells were resuspended in serum-free medium, mixed with Matrigel Basement Membrane Matrix (Corning, Corning, NY) at a 1:1 ratio, and injected subcutaneously into the flanks of female NOD.CB17/AlhnRj-Prkdc^SCID/Rj mice 8 to 10 weeks of age (Janvier Labs, Le Genest-Saint-Isle, France). After tumor engraftment, mice were randomly assigned to be administered CFZ (2 mg/kg) IV, subasumstat (25 mg/kg) IV, a combination of both, or vehicle control twice per week. Treatment was performed for a total of 7 days. Tumor growth was monitored by caliper measurements. Mice were housed under specific pathogen-free conditions, and animal experiments were conducted in accordance with the local ethical guidelines and approved by the responsible regional authorities (District Government of Upper Bavaria; application no. ROB-55.2-2532.Vet_02-17-230).

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Heynen G.J., Baumgartner F., Heider M., Patra U., Holz M., Braune J., Kaiser M., Schäffer I., Bamopoulos S.A., Ramberger E., Murgai A., Ng Y.L., Demel U.M., Laue D., Liebig S., Krüger J., Janz M., Nogai A., Schick M., Mertins P., Müller S., Bassermann F., Krönke J., Keller U, & Wirth M. (2022). SUMOylation inhibition overcomes proteasome inhibitor resistance in multiple myeloma. Blood Advances, 7(4), 469-481.

Publication 2022

Matrigel Basement Membrane Matrix NOD.CB17/AlhnRj-PrkdcSCID/Rj mice

Basement membrane Cells Female Matrigel Mice Nod mice Serum Specific pathogen free Tumor Xenograft

Corresponding Organization : Max Delbrück Center

Other organizations : Technical University of Munich, Goethe University Frankfurt

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Variable analysis

independent variables

Administration of CFZ (2 mg/kg) IV
Administration of subasumstat (25 mg/kg) IV
Combination of CFZ and subasumstat

dependent variables

Tumor growth

control variables

Female NOD.CB17/AlhnRj-Prkdc^SCID^/Rj mice aged 8 to 10 weeks
Subcutaneous injection of 1.0 × 10^7 MM cells resuspended in serum-free medium mixed with Matrigel Basement Membrane Matrix at a 1:1 ratio
Administration of vehicle control twice per week

controls

Negative control: Vehicle control

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