C57BL/6J (B6), PWD/PhJ (PWD), and B6.ChrPWD consomic mice were purchased from Jackson Laboratories (Bar Harbor, ME, USA), then bred and housed in a single room within the vivarium at the Larner College of Medicine at the University of Vermont for five or more generations. The experimental procedures used in this study were approved by the Animal Care and Use Committee of the University of Vermont.
To ensure their correct identity and to enhance rigor and reproducibility of these studies, B6.ChrPWD consomic mice were subjected to genome-wide SNP genotyping using DartMouse genotyping services (Dartmouth College, NH, USA), as previously described by us 22 (link). All mice used in this study were of the expected genotypes, with the following exception. Chr17SPWD mice were found to carry a homozygous B6-derived interval between 30 and 45 Mb on Chr17, encompassing H-2. Chr17FPWD mice with the full PWD-derived Chr17 were generously provided by Dr. Jiri Forejt (Institute of Molecular Genetics of the ASCR, Czech Republic) and housed in the vivarium at UVM for two or more generations prior to experimentation.
To ensure their correct identity and to enhance rigor and reproducibility of these studies, B6.ChrPWD consomic mice were subjected to genome-wide SNP genotyping using DartMouse genotyping services (Dartmouth College, NH, USA), as previously described by us 22 (link). All mice used in this study were of the expected genotypes, with the following exception. Chr17SPWD mice were found to carry a homozygous B6-derived interval between 30 and 45 Mb on Chr17, encompassing H-2. Chr17FPWD mice with the full PWD-derived Chr17 were generously provided by Dr. Jiri Forejt (Institute of Molecular Genetics of the ASCR, Czech Republic) and housed in the vivarium at UVM for two or more generations prior to experimentation.
