Glioma Tumorsphere Culture and Chemotherapeutic Testing

This study was conducted in accordance with Declaration of Helsinki. All tumor and blood samples were collected with patient consent under protocols approved at Yokohama City University (YCU) Hospital and Massachusetts General Hospital (MGH) Institutional Review Boards. To create glioma tumorsphere lines, fresh tumor specimens were obtained from surgery and enzymatically dissociated with 0.1% of Trypsin and DNase. Tumorsphere lines were cultured in serum-free neural stem cell medium, as previously described (3 (link),25 (link),28 (link)). All tumorsphere lines were cryopreserved less than passage 3 to use for in vitro experiments. Temozolomide (TMZ, Sigma), Nimustine (ACNU, Tokyo Chemical Industry), Lomustine (CCNU), Procarbazine (Sigma), Vincristine (Sigma), FK866 (Sigma), LY294002 (Sellek), GDC-0068 (Cayman), BYL719 (Cayman), Everolimus (Cayman), and AGI-5198 (Sigma) were used.

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Tateishi K., Nakamura T., Juratli T.A., Williams E.A., Matsushita Y., Miyake S., Nishi M., Miller J.J., Tummala S.S., Fink A.L., Lelic N., Koerner M.V., Miyake Y., Sasame J., Fujimoto K., Tanaka T., Minamimoto R., Matsunaga S., Mukaihara S., Shuto T., Taguchi H., Udaka N., Murata H., Ryo A., Yamanaka S., Curry W.T., Dias-Santagata D., Yamamoto T., Ichimura K., Batchelor T.T., Chi A.S., Iafrate A.J., Wakimoto H, & Cahill D.P. (2019). PI3K/AKT/mTOR pathway alterations promote malignant progression and xenograft formation in oligodendroglial tumors. Clinical cancer research : an official journal of the American Association for Cancer Research, 25(14), 4375-4387.

Publication 2019

Temozolomide (TMZ, Vincristine FK866 GDC-0068 BYL719 Everolimus AGI-5198

Acnu Agi 5198 Blood Byl719 Cayman Ccnu Cultured medium Dnase Everolimus Gdc 0068 Glioma Institutional review boards Lomustine Ly294002 Neural stem cell Patient Procarbazine Serum Surgery Trypsin 1 Tumor Vincristine

Corresponding Organization :

Other organizations : Yokohama City University, Massachusetts General Hospital, Harvard University, Yokohama City University Hospital, Center for Neuro-Oncology, National Center for Global Health and Medicine, Yokohama Rosai Hospital, Fujisawa City Hospital, NYU Langone Health

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Variable analysis

independent variables

Temozolomide (TMZ)
Nimustine (ACNU)
Lomustine (CCNU)
Procarbazine
Vincristine
FK866
LY294002
GDC-0068
BYL719
Everolimus
AGI-5198

dependent variables

Tumor cell proliferation
Tumor cell survival

control variables

Passage number of tumorsphere lines (less than 3)
Cryopreservation of tumorsphere lines

controls

Positive controls: Not specified
Negative controls: Not specified

Annotations

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