Neutrophil Differentiation Assay

The differentiation of PLB-985 and PLB-985-KO cells into the neutrophil-like phenotype was performed as reported (45 (link)). The cell surface expression of CD11b was quantified to confirm the differentiation of PLB-985 and PLB-985-KO to neutrophils, as described (43 (link), 46 (link)), using antihuman CD11b monoclonal antibody (ICRF44; eBioscience) and antimouse/Alexa Fluor 647 (BioLegend). The fluorescence was analyzed by flow cytometry (Attune NxT, ThermoFisher Scientific).

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Hernandez-Morfa M., Reinoso-Vizcaíno N.M., Olivero N.B., Zappia V.E., Cortes P.R., Jaime A, & Echenique J. (2022). Host Cell Oxidative Stress Promotes Intracellular Fluoroquinolone Persisters of Streptococcus pneumoniae. Microbiology Spectrum, 10(6), e04364-22.

Publication 2022

ICRF44 Attune NxT

Alexa fluor 647 Cd11b Flow cytometry Fluorescence Monoclonal antibody Neutrophils Phenotype

Corresponding Organization : Research Centre in Biological Chemistry of Córdoba

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Variable analysis

independent variables

Differentiation of PLB-985 and PLB-985-KO cells into the neutrophil-like phenotype

dependent variables

Cell surface expression of CD11b

control variables

Differentiation protocol as reported (45)
Use of antihuman CD11b monoclonal antibody (ICRF44; eBioscience) and antimouse/Alexa Fluor 647 (BioLegend) to quantify CD11b expression
Flow cytometry analysis (Attune NxT, ThermoFisher Scientific)

controls

Positive control: Not explicitly mentioned
Negative control: Not explicitly mentioned

Annotations

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