Cytotoxicity Evaluation of Cell Lines

The human colorectal adenocarcinoma cell line HT-29 (Cat. no: 85061109), human gastric carcinoma Hs 746T cell line (ATCC^® HTB-135™), human colon cancer Caco-2 cell line (ATCC^® HTB-37™), and human normal colon mucosa CCD 841 CoN cell line (ATCC^® CRL-1790™) were used in this study and cultured according to the method that was previously described in detail in this report [23 (link)]. The cell viability and metabolic activity were determined using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) colorimetric assay [78 (link)]. The first cytotoxic dose (IC₁₀), the median effective concentration (IC₅₀), and the lethal dose (IC₉₀) were calculated on the basis of the MTT results.

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Kowalczewski P.Ł., Olejnik A., Rybicka I., Zielińska-Dawidziak M., Białas W, & Lewandowicz G. (2021). Membrane Filtration-Assisted Enzymatic Hydrolysis Affects the Biological Activity of Potato Juice. Molecules, 26(4), 852.

Publication 2021

HTB-135 HTB-37 CRL-1790

Adenocarcinoma Assay Bromide Carcinoma Cell viability Colon Colon cancer Colorimetric Cultured method Gastric Ht 29 Human Mucosa Normal cell line

Corresponding Organization : University of Life Sciences in Poznań

Other organizations : Poznań University of Economics and Business

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Variable analysis

independent variables

Cell lines used: HT-29 (human colorectal adenocarcinoma), Hs 746T (human gastric carcinoma), Caco-2 (human colon cancer), and CCD 841 CoN (human normal colon mucosa)

dependent variables

Cell viability and metabolic activity determined using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) colorimetric assay
First cytotoxic dose (IC10), median effective concentration (IC50), and lethal dose (IC90) calculated based on MTT results

control variables

Cell culture conditions and methods previously described in detail in the referenced report [23]

controls

No positive or negative controls were explicitly mentioned in the provided information.

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