Electrophysiological Recordings in Bengalese Finches
All procedures described below were approved by The Institutional Animal Care and Use Committee at Emory University. As described previously (Srivastava, Elemans, and Sober 2015 (link); Zia et al. 2020 (link); Zia et al. 2018 ), adult male Bengalese finches (>90 d old) were anesthetized using intramuscular injections of 40 mg/kg ketamine and 3 mg/kg midazolam injected and anesthesia was maintained using 1–5% isoflurane in oxygen gas. To record from the expiratory (respiratory) muscles, an incision was made dorsal to the leg attachment and rostral to the pubic bone and the electrode array was placed on the muscle surface using the “epimysial” approach described above. To record from syringeal (vocal) muscles, the vocal organ was accessed for electrode implantation via a midline incision into the intraclavicular air sac as described previously (Srivastava, Elemans, and Sober 2015 (link)) to provide access to the ventral syringeal (VS) muscle located on the ventral portion of the syrinx near the midline.
Chung B., Zia M., Thomas K.A., Michaels J.A., Jacob A., Pack A., Williams M.J., Nagapudi K., Teng L.H., Arrambide E., Ouellette L., Oey N., Gibbs R., Anschutz P., Lu J., Wu Y., Kashefi M., Oya T., Kersten R., Mosberger A.C., O’Connell S., Wang R., Marques H., Mendes A.R., Lenschow C., Kondakath G., Kim J.J., Olson W., Quinn K.N., Perkins P., Gatto G., Thanawalla A., Coltman S., Kim T., Smith T., Binder-Markey B., Zaback M., Thompson C.K., Giszter S., Person A., Goulding M., Azim E., Thakor N., O’Connor D., Trimmer B., Lima S.Q., Carey M.R., Pandarinath C., Costa R.M., Pruszynski J.A., Bakir M, & Sober S.J. (2023). Myomatrix arrays for high-definition muscle recording. bioRxiv.
Other organizations :
Georgia Institute of Technology, Western University, Columbia University, Champalimaud Foundation, Otto-von-Guericke University Magdeburg, Tufts University, Johns Hopkins University, Johns Hopkins Medicine, University Hospital Cologne, Salk Institute for Biological Studies, University of Colorado Anschutz Medical Campus, Drexel University, Temple University, Allen Institute
Anesthesia administration (intramuscular injections of 40 mg/kg ketamine and 3 mg/kg midazolam, and maintenance using 1–5% isoflurane in oxygen gas)
dependent variables
Electrical activity recorded from expiratory (respiratory) muscles
Electrical activity recorded from syringeal (vocal) muscles
control variables
Adult male Bengalese finches (>90 d old)
Electrode array placement on the muscle surface using the "epimysial" approach
positive controls
None mentioned
negative controls
None mentioned
Annotations
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