Antibodies employed for the migratory scWB study include rabbit anti-EphA219 (link),20 (link) (1:10, 6997S, Cell Signaling, with anti-rabbit secondary antibody conjugated with Alexa-Fluor 647), mouse anti-STAT321 (link),22 (link) (1:10, 9139S, Cell Signaling, with anti-mouse secondary antibody conjugated with Alexa-Fluor 488), mouse anti-Nestin23 (link),24 (link) (1:10, MAB5326, EMD-Millipore, with anti-mouse secondary antibody conjugated with Alexa-Fluor 488), rabbit anti-β-tubulin17 (link),25 (link) (1:10, ab6046, Abcam, with anti-rabbit secondary antibody conjugated with Alexa-Fluor 647).
Protocol detail
Single-cell Western Blotting with Migratory Antibodies
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Corresponding Organization : University of California, Berkeley
Other organizations : University of California System
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Variable analysis
independent variables
- Rabbit anti-EphA2
- Mouse anti-STAT3
- Mouse anti-Nestin
- Rabbit anti-β-tubulin
- Not explicitly mentioned
- Not explicitly mentioned
- Positive control: None specified
- Negative control: None specified
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