Characterization of Waldenstrom's Macroglobulinemia Cell Lines

The IgM secreting cell line BCWM.1 (17 (link), 18 ) was a kind gift from Dr. Steven Treon (Dana Farber Cancer Institute, Boston, MA). MWCL-1 cells (19 ) were a kind gift from Dr. Stephen Ansell (Mayo Clinic, Rochester, MN) and RPCI-WM1 cells (20 (link)) were kindly provided by Dr. Asher Chanan-Khan (Mayo Clinic, Jacksonville, FL). All cells were grown in RPMI-1640 supplemented with 10% FBS and penicillin/streptomycin. The GLI1/2 inhibitor (GANT61) and HH inhibitor (Cyclopamine) were obtained from EMD Millipore (Billerica, MA). The pan-caspase inhibitor (Q-VD-OPh) and all primers were obtained from Sigma-Aldrich (St. Louis, MO). β-actin antibody was obtained from Novus Biologicals (Littleton, CO). IL-6Rα antibody for western blot was from Santa Cruz Biotechnology (Dallas, TX). FITC-conjugated Annexin V was obtained from BD Biosciences (San Jose, CA) and propidium iodide was from Sigma-Aldrich.

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Jackson D.A., Smith T.D., Amarsaikhan N., Han W., Neil M.S., Boi S.K., Vrabel A.M., Tolosa E.J., Almada L.L., Fernandez-Zapico M.E, & Elsawa S.F. (2015). Modulation of the IL-6 Receptor α Underlies GLI2-mediated regulation of Immunoglobulin Secretion in Waldenström macroglobulinemia cells. Journal of immunology (Baltimore, Md. : 1950), 195(6), 2908-2916.

Publication 2015

GANT61 Cyclopamine pan-caspase inhibitor (Q-VD-OPh) β-actin antibody FITC-conjugated Annexin V propidium iodide

Actin Annexin v Antibody Biologicals Cancer Caspase inhibitor Cell line Cells Cyclopamine Fitc Gant61 Novus Penicillin Primers Propidium iodide Q vd oph Streptomycin Western blot

Corresponding Organization : Northern Illinois University

Other organizations : Novel (United States), Mayo Clinic

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Variable analysis

independent variables

GLI1/2 inhibitor (GANT61)
HH inhibitor (Cyclopamine)

dependent variables

Cell growth/proliferation
Apoptosis

control variables

Cell culture media (RPMI-1640 supplemented with 10% FBS and penicillin/streptomycin)
Pan-caspase inhibitor (Q-VD-OPh)

controls

Positive control: BCWM.1, MWCL-1, and RPCI-WM1 cell lines
Negative control: Not specified

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