Cell Viability Assay for OVCA Tumor Cultures

The cell viability of OVCA ex vivo tumor cultures was determined in accordance with a protocol previously described by our group.28 (link) Briefly, cell viability was verified at days 3 and 6 or 7 by incubating 2–3 hours 50 μL of CellTiter 96 AQueous One Solution Proliferation Assay reagent (Promega, Wisconsin, USA). Absorbance was read at 492 nm using a Fluostar OPTIMA analyzer (BMG Labtech, Offenburg, Germany). Each time data point was normalized to the vehicle control group.

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Santos J.M., Heiniö C., Cervera-Carrascon V., Quixabeira D.C., Siurala M., Havunen R., Butzow R., Zafar S., de Gruijl T., Lassus H., Kanerva A, & Hemminki A. (2020). Oncolytic adenovirus shapes the ovarian tumor microenvironment for potent tumor-infiltrating lymphocyte tumor reactivity. Journal for Immunotherapy of Cancer, 8(1), e000188.

Publication 2020

CellTiter 96 AQueous One Solution Proliferation Assay reagent Fluostar OPTIMA analyzer

Assay Cell cultures Cell viability Promega Tumor

Corresponding Organization :

Other organizations : Gene Therapy Laboratory, University of Helsinki, Helsinki University Hospital

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Variable analysis

independent variables

Days of cell culture (3 and 6 or 7)

dependent variables

Cell viability

control variables

Vehicle control group

controls

Vehicle control group

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