At 8–12 weeks of age, mice were anesthetized with an intramuscular injection of ketamine (80 mg/kg) and xylazine (8 mg/kg) cocktail and ovaries were removed bilaterally as previously described [25 (link)]. All animals were given two weeks to recover prior to infection. Recombinant amphiregulin (10μg; R&D) was administered intraperitoneally every other day using saline as the vehicle.
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