Imaging Salt Stress Response in Bacteria

Wildtype and ΔyciB cells were grown in LB containing low salt (75mM NaCl) before being transferred to LB with high salt (600mM NaCl) and grown for 6-7 generations before being fixed as described before 54 (link). Fixed cells were imaged on a Nikon Eclipse TE inverted fluorescence microscope with a 60X (NA 1.40) oil-immersion objective. Images were collected using the DS-Qi2 Mono Digital Microscope Camera and the NIS-Elements AR (Advanced Research) software (Nikon).

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Kritikos G., Banzhaf M., Herrera-Dominguez L., Koumoutsi A., Wartel M., Zietek M, & Typas A. (2017). A tool named Iris for versatile high-throughput phenotyping in microorganisms. Nature microbiology, 2, 17014.

Publication 2017

Eclipse TE

Camera Cells Fluorescence microscope Immersion Microscope Nacl Salt

Corresponding Organization :

Other organizations : European Molecular Biology Laboratory

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Protocol cited in 2 other protocols

Variable analysis

independent variables

Presence or absence of yciB gene (Wildtype vs. ΔyciB)

dependent variables

Cell morphology or other phenotypic changes

control variables

Growth medium (LB)
Low salt concentration (75mM NaCl) before treatment
High salt concentration (600mM NaCl) during treatment
Growth duration (6-7 generations)
Imaging method (Nikon Eclipse TE inverted fluorescence microscope with 60X, NA 1.40 oil-immersion objective)
Imaging software (NIS-Elements AR)

controls

Not explicitly mentioned
Not explicitly mentioned

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