Xie et al.13 (link). reported that an EST sequence of HOT is over-expressed in the THR strain of B. tabaci. The 3′ and 5′ RACE reactions were constructed using the SMARTTM RACE amplification kit (Clontech, USA) according to the manufacturer’s protocol. All primers used are listed in Table 2 . The PCR products were cloned into the pMD18-T simple vector (TaKaRa, Japan). At least 10 clones from both the 5′- and 3′-RACE libraries were sequenced using both M13 forward and M13 reverse primers.
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