Bioluminescence Imaging of LPS-Induced Inflammation

Mice were imaged on days 3–5 after adenovirus delivery. Mice were fasted for 12 h starting at Zeitgeber time (ZT) 12 and injected intraperitoneally with LPS (30 mg kg⁻¹) for 1 h. Before imaging, mice were injected intraperitoneally with 50 mg kg⁻¹ Nembutal (Abbott Laboratories, Lake Bluff, IL, USA) and 100 mg kg⁻¹ sterile firefly D-luciferin (Qianchen, Shanghai, China). Mice were imaged using the IVIS 100 Imaging System (PerkinElmer, Waltham, MA, USA) and image data were analyzed using Living Image software (Xenogen, Hudson, NY, USA) as described [11 (link)]. Liver samples were taken immediately after imaging and lysates were subjected to β-galactosidase assay to ensure the equal level of adenoviral infection.