Validating Gene Expression Signatures

Additional validation groups (Supplementary Methods and eTable 1 in the Supplemental Appendix) included children with meningococcal sepsis,16 inflammatory diseases (Juvenile Idiopathic Arthritis and Henoch-Schönlein purpura) and published gene expression datasets which compared bacterial infection with viral infection,12 (link),15 (link),17 (link) or inflammatory disease.18 (link) Healthy children were recruited from out-patient departments. Data from healthy controls were not utilized in identification or validation of gene expression signatures, and were only used for interpretation of direction of gene regulation.

Partial Protocol Preview
This section provides a glimpse into the protocol.
The remaining content is hidden due to licensing restrictions, but the full text is available at the following link: Access Free Full Text.

Herberg J.A., Kaforou M., Wright V.J., Shailes H., Eleftherohorinou H., Hoggart C.J., Cebey-Lopez M., Carter M.J., Janes V.A., Gormley S., Shimizu C., Tremoulet A.H., Barendregt A.M., Salas A., Kanegaye J., Pollard A.J., Faust S.N., Patel S., Kuijpers T., Martinon-Torres F., Burns J.C., Coin L.J, & Levin M. (2016). Diagnostic test accuracy of a 2-transcript host RNA signature for discriminating bacterial vs viral infection in febrile children. JAMA, 316(8), 835-845.

Publication 2016

Bacterial viral infection Children Gene expression Gene regulation Henoch schönlein purpura Infection Inflammatory Juvenile idiopathic arthritis Meningococcal Out patient Sepsis

Corresponding Organization :

Other organizations : Imperial College London, Instituto de Investigación Sanitaria de Santiago, Universidade de Santiago de Compostela, Rady Children's Hospital-San Diego, University of California, San Diego, University of Amsterdam, Emma Kinderziekenhuis, University of Oxford, University Hospital Southampton NHS Foundation Trust, University of Southampton, Wellcome Trust, Sanquin, University of Queensland

Top 5 similar protocols

Protocol cited in 17 other protocols

Variable analysis

independent variables

Meningococcal sepsis
Inflammatory diseases (Juvenile Idiopathic Arthritis and Henoch-Schönlein purpura)
Bacterial infection
Viral infection
Inflammatory disease

dependent variables

Not explicitly mentioned

control variables

Healthy children recruited from out-patient departments

controls

Positive control: Not specified
Negative control: Healthy children recruited from out-patient departments

Annotations

Based on most similar protocols

Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.

As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!