Three bacterial cultures with a broad range of genomic G+C content, for which finished genomes are available in public databases, were employed in the optimization of WGA-X: Prochlorococcus marinus, Eschericia coli, and Meiothermus ruber (Supplementary Table 1 ). Cultures of two marine unicellular algae, Thalassiosira pseudonana and Ostreococcus lucimarinus (Supplementary Table 1 ) were also used in the subsequent benchmarking of optimized WGA-X and its comparison to MDA.
For marine prokaryote and extracellular particle analyses, the Gulf of Maine surface water was collected from 1 m depth in Boothbay Harbor, Maine (43°50′39.87″ N, 69°38′27.49″ W) on 15 June, 2011. One ml aliquots were amended with 5% glycerol and 1× TE buffer (all final concentrations), and stored at −80 °C until further analysis. The marine microalgae sample was collected from the same location on 16 September, 2009 and cryopreserved with 6% glycine betaine and 1× TE buffer (all final concentrations) at −80 °C. The soil sample was collected from 0–10 cm depth in a residential garden in Nobleboro, Maine (44°5′48.10″ N, 69°29′10.56″ W) on 5 May, 2015. Approximately 5 g of the soil sample were mixed with 30 ml sterile-filtered PBS, vortexed for 30 s at maximum speed, and centrifuged for 30 s at 2000 rpm (800×g). The obtained supernatant was used for cell sorting within 30 min, and processed as described above.
For marine prokaryote and extracellular particle analyses, the Gulf of Maine surface water was collected from 1 m depth in Boothbay Harbor, Maine (43°50′39.87″ N, 69°38′27.49″ W) on 15 June, 2011. One ml aliquots were amended with 5% glycerol and 1× TE buffer (all final concentrations), and stored at −80 °C until further analysis. The marine microalgae sample was collected from the same location on 16 September, 2009 and cryopreserved with 6% glycine betaine and 1× TE buffer (all final concentrations) at −80 °C. The soil sample was collected from 0–10 cm depth in a residential garden in Nobleboro, Maine (44°5′48.10″ N, 69°29′10.56″ W) on 5 May, 2015. Approximately 5 g of the soil sample were mixed with 30 ml sterile-filtered PBS, vortexed for 30 s at maximum speed, and centrifuged for 30 s at 2000 rpm (800×g). The obtained supernatant was used for cell sorting within 30 min, and processed as described above.
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