Immunofluorescence Staining of GBM6 Cells

GBM6 cells were grown on 8-well chamber slides (Labtek, Thermo Fisher Scientific), precoated for 1 h with poly-DL-ornithine (Sigma-Aldrich) (10µg/ml), to be treated for 48 h with BAL27862. As previously described [19 (link)], cells were incubated with an anti- α-tubulin (clone DM1A; Sigma-Aldrich) primary antibody, and then with Alexa488 secondary antibody (Invitrogen). Staining was observed using either a Leica DM-IRBE microscope. Images were acquired using Metamoph software and were processed using Image J software.

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Bergès R., Tchoghandjian A., Sergé A., Honoré S., Figarella-Branger D., Bachmann F., Lane H.A, & Braguer D. (2020). EB1-dependent long survival of glioblastoma-grafted mice with the oral tubulin-binder BAL101553 is associated with inhibition of tumor angiogenesis. Oncotarget, 11(8), 759-774.

Publication 2020

Labtek poly-DL-ornithine anti- α-tubulin (clone DM1A; Alexa488 secondary antibody Leica DM-IRBE microscope

Antibody Bal27862 Cells Clone Microscope Ornithine Poly α tubulin

Corresponding Organization :

Other organizations : Centre National de la Recherche Scientifique, Institut de Neurophysiopathologie, Inserm, Institut Paoli-Calmettes, Hôpital de la Timone, Basilea Pharmaceutica (Switzerland)

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Variable analysis

independent variables

BAL27862 treatment
Duration of BAL27862 treatment (48 h)

dependent variables

α-tubulin expression and localization

control variables

Cell line (GBM6 cells)
Cell culture substrate (8-well chamber slides, precoated with poly-DL-ornithine)
Primary antibody (anti-α-tubulin, clone DM1A)
Secondary antibody (Alexa488)

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