Monocyte-derived dendritic cell culture
Corresponding Organization :
Other organizations : University of St Andrews
Variable analysis
- Treatment with 100 ng/ml of bacterial LPS
- Cellular response of moDCs
- Isolation of PBMCs over a ficoll gradient
- Differentiation of PBMCs into moDCs using IL-4 and GM-CSF
- Cell culture in sterile RPMI containing 10% fetal calf serum
- Cell cultivation for up to 24 hours
- Untreated moDCs as negative control
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