Mesenchymal Stem Cell Surface Marker Profiling

Flow cytometry was used to examine the surface protein expression of mesenchymal stem cell markers^{44 (link),45 (link)}. The cells were detached using trypsin/EDTA solution to obtain a single cell suspension. The cells were immunostained with primary antibodies conjugated with fluorescent dye, comprising CD44 (Cat No. AM310‐10M, BioGenex, Fremont CA, USA), CD45 (Cat No. AM111‐10M, BioGenex), CD73 (Cat No. 21270733, ImmunoTools, Friesoythe, Germany), CD90 (Cat No. 21270906, ImmunoTools), and CD105 (Cat No. 21271054, ImmunoTools). Flow cytometry analysis was performed using a FACS^Calibur Flow cytometer (BD Biosciences, NJ, USA).