Protocol detail

Drosophila Neuronal Developmental Protocols

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worGal4, UAS-cherry::Jupiter, Sqh::GFP, Histone2A::mRFP1 (Bloomington stock center), UAS-mCherry::CAAX (Bloomington stock center), UAS-Pp1-87B-HA (Bloomington stock center), UAS-Tum::Venus⁴¹. UAS-Ran-Q69L, UAS-Ran-T24N^{62 (link)}. UAS-Gap43::mEos^{30 (link)}. Sqh::mCherry^{63 (link)}. pUAST-attB-ALD-Rock^CA::VhhGFP4::HA, pUAST-attB-ALD-Rock^CA::mCherry (this study).
Transgenes were expressed using the neuroblast-specific driver worGal4^{64 (link)}.
Zipper::mDendra2 MiMIC^{65 (link)} line: Mi02518 was crossed to phiC31 integrase (expressed under the vasa promotor; Bloomington stock center) and the resulting progeny were injected with the mDendra2 exchange cassette^{65 (link)}. Injections were performed by BestGene. Positive lines were initially screened for loss of yellow body marker and tested for the expression of Zipper::mDendra2.

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Roubinet C., Tsankova A., Pham T.T., Monnard A., Caussinus E., Affolter M, & Cabernard C. (2017). Spatio-temporally separated cortical flows and spindle geometry establish physical asymmetry in fly neural stem cells. Nature Communications, 8, 1383.

Publication 2017

UAS-mCherry::CAAX

Body Cherry Integrase Transgenes

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Variable analysis

independent variables

WorGal4
UAS-Ran-Q69L
UAS-Ran-T24N
PUAST-attB-ALD-Rock^CA::VhhGFP4::HA
PUAST-attB-ALD-Rock^CA::mCherry

dependent variables

Cherry::Jupiter
Sqh::GFP
Histone2A::mRFP1
UAS-mCherry::CAAX
UAS-Pp1-87B-HA
UAS-Tum::Venus
UAS-Gap43::mEos
Sqh::mCherry
Zipper::mDendra2

control variables

Bloomington stock center

positive controls

Zipper::mDendra2 MiMIC line: Mi02518 crossed to phiC31 integrase (expressed under the vasa promotor)

negative controls

Not explicitly mentioned

Annotations

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