Panel-based next generation sequencing was conducted in cases with DNA that passed quality controls. Tumor DNA was sequenced using a home-made panel of genes as previously described [23 (link)]. For each tumor sample, a library of all coding exons and intron–exon boundaries of a panel of 794 target genes (Additional file 1: Table S1) was constructed using the SureSelect enrichment system (Agilent Technologies, Santa Clara, CA, USA). Sequencing was carried out using the Illumina NextSeq500 device (San Diego, CA, USA), according to the manufacturer’s instruction at a median depth of 162×.
Nigon E., Lefeuvre-Plesse C., Martinez A., Chauleur C., Lortholary A., Favier L., Bats A.S., Guille A., AdélaÏde J., Finetti P., de Casteljac V., Provansal M., Mamessier E., Bertucci F., Ray-Coquard I, & Sabatier R. (2023). Clinical, pathological, and comprehensive molecular analysis of the uterine clear cell carcinoma: a retrospective national study from TMRG and GINECO network. Journal of Translational Medicine, 21, 408.
Other organizations :
Inserm, Institut Paoli-Calmettes, Centre National de la Recherche Scientifique, Centre Eugène Marquis, Institut universitaire du cancer de Toulouse Oncopole, Hôpital privé du Confluent, Centre Georges François Leclerc, Hôpital Européen, Assistance Publique – Hôpitaux de Paris, Hôpital Européen Georges-Pompidou, Université Claude Bernard Lyon 1, Centre Léon Bérard
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