Western blot analysis was done as described previously50 (link). Antibodies were obtained from several providers. Cleaved caspase-3 (9661), Cleaved caspase-9 (7237), Caspase-3 (9662), Caspase-9 (9508) and p65 (8242) were from Cell Signaling (Danvers, MA, USA), β-actin (sc-130300), PCNA (sc-25280), Bax (sc-7480), p50 (sc-114), MMP-2 (sc-13595), MMP-3 (sc-6839), MMP-13 (sc-12363), normal mouse IgG (sc-2025) and MMP-9 (sc-127590 were purchased from Santacruz (Dallas, TX, USA) and IL-32 (513601) was obtained from BioLegend, (San Diego, CA USA). TIMP3 (ab39184) and DNMT1 (ab19905) were obtained from Abcam (Cambridge, MA, USA).
Free full text: Click here