Cellular Reprogramming via Exosomal Delivery

Example 7

Since the genetic material may be delivered by the exosomes, it has been hypothesized that the exosomes secreted from the cells cultured in a human ES medium may change the properties of surrounding cells or untreated cells. To verify this, the exosomes were extracted from the 2-day cultured medium of cells treated with ultrasonic wave cultured in the human ES medium environment, and the exosome extract was mixed and cultured for 6 days in a process of culturing the untreated cells in the human ES medium and a fibroblast culture medium, DMEM.

As a result, spheroid was produced in a group added with exosomes (FIG. 7A), and as a result of verifying the Oct4 expression in the cells, the expression of a pluripotent marker, Oct4, was observed (FIG. 7B). This indicates that the delivery of the genetic material by the exosomes may induce the cell reprogramming.