Western Blotting of NF-κB Pathway

Western blotting was performed as previously described (Chen et al., 2002 (link); Xiao et al., 2019 (link)). Antibodies anti-myeloid differentiation primary response gene 88 (MyD88) (D80F5; #4283), anti-phospho-IKKα/β (Ser176/180) (16A6; #2697), anti-IKKα (#2682), Phospho-IKKα/β (Ser176/180) (16A6, #2697), anti-IκBα (44D4,#4812), anti-Phospho-IκBα (Ser32/36) (5A5, #9246), anti-NF-κB p65 (D14E12, #8242) and anti-Phospho-NF-κB p65 (Ser536) (93H1, #3033) were purchased from Cell Signaling Technology.

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Su J., Guo K., Huang M., Liu Y., Zhang J., Sun L., Li D., Pang K.L., Wang G., Chen L., Liu Z., Chen Y., Chen Q, & Huang L. (2019). Fucoxanthin, a Marine Xanthophyll Isolated From Conticribra weissflogii ND-8: Preventive Anti-Inflammatory Effect in a Mouse Model of Sepsis. Frontiers in Pharmacology, 10, 906.

Publication 2019

MyD88) (D80F5; anti-phospho-IKKα/β (Ser176/180) (16A6; anti-IκBα (44D4,# anti-Phospho-IκBα (Ser32/36) (5A5, anti-NF-κB p65 (D14E12, anti-Phospho-NF-κB p65 (Ser536) (93H1,

Antibodies gene Ikkα Nf κb p65 Phospho

Corresponding Organization : Fujian Institute of Oceanography

Other organizations : The University of Texas Southwestern Medical Center, National Taiwan Ocean University, Institute of Oceanology, Qingdao National Laboratory for Marine Science and Technology, Fudan University, Huashan Hospital, Fujian Fisheries Research Institute

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Variable analysis

independent variables

None explicitly mentioned

dependent variables

Levels of myeloid differentiation primary response gene 88 (MyD88)
Levels of phosphorylated IKKα/β (Ser176/180)
Levels of IKKα
Levels of phosphorylated IKKα/β (Ser176/180)
Levels of IκBα
Levels of phosphorylated IκBα (Ser32/36)
Levels of NF-κB p65
Levels of phosphorylated NF-κB p65 (Ser536)

control variables

None explicitly mentioned

controls

Positive control: Not specified
Negative control: Not specified

Annotations

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