VEGFR2 Inhibition Impacts Cell Viability

The effects of the inhibition of VEGF signaling on cell viability and apoptosis were investigated using a selective inhibitor of VEGFR2 (ZM323881 hydrochloride, Tocris, Bristol, UK) diluted to 1 μM in modified DMEM. Control cells were treated with the same concentration of the VEGFR2 inhibitor diluent alone (DMSO). Cells were treated just before light exposure. Viability and apoptosis were assessed 6 h after the end of light exposure using the ApoLive-Glo^™ Multiplex Assay (Promega) as previously described [45 (link)].

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Marie M., Gondouin P., Pagan D., Barrau C., Villette T., Sahel J, & Picaud S. (2019). Blue-violet light decreases VEGFa production in an in vitro model of AMD. PLoS ONE, 14(10), e0223839.

Publication 2019

ZM323881 hydrochloride ApoLive-Glo™ Multiplex Assay

Apoptosis Assay Cell viability Cells Dmso Inhibition Light Promega Vegf Vegfr2 Zm323881

Corresponding Organization : Centre National de la Recherche Scientifique

Other organizations : Essilor (France), University of Pittsburgh

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Variable analysis

independent variables

VEGFR2 inhibitor (ZM323881 hydrochloride)

dependent variables

Cell viability
Apoptosis

control variables

DMSO (VEGFR2 inhibitor diluent)

controls

Positive control: Cells treated with VEGFR2 inhibitor (ZM323881 hydrochloride)
Negative control: Cells treated with DMSO (VEGFR2 inhibitor diluent)

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