Microstructural Analysis of Bone Callus

At 4 weeks postoperatively, five rats from each group were killed and the sample harvested in preparation for the radiographic and histological studies. Bone samples were examined with a μCT-100 scanner (Scanco Medical, Switzerland) with a resolution of 24 μm and analyzed using the accompanying supporting analysis and three-dimentional imaging software to evaluate callus formation. The bone volume fraction (BV/TV) and the trabecular thickness (Tb.Th) were calculated by standard three-dimensional microstructural analysis [25 (link), 26 (link)].

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Gao X., Wang J., Wang Y., Li W, & Pan Z. (2023). The m6A Reader YTHDF1 Accelerates the Osteogenesis of Bone Marrow Mesenchymal Stem Cells Partly via Activation of the Autophagy Signaling Pathway. Stem Cells International, 2023, 5563568.

Publication 2023

μCT-100 scanner

Bone Callus Radiographic Rats Trabecular

Corresponding Organization : Zhejiang Lab

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Variable analysis

independent variables

Postoperative time (4 weeks)

dependent variables

Bone volume fraction (BV/TV)
Trabecular thickness (Tb.Th)

control variables

Bone samples examined with a μCT-100 scanner (Scanco Medical, Switzerland) with a resolution of 24 μm
Bone samples analyzed using the accompanying supporting analysis and three-dimensional imaging software

controls

Positive control: Not explicitly mentioned
Negative control: Not explicitly mentioned

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