ACKR3 Phosphosite Mapping by MS

Phosphosite mapping was performed at the Purdue University Proteomics Facility. Briefly, ACKR3 in LMNG or nanodisc was first phosphorylated by GRK2 and GRK5 and then digested with trypsin. The fragments were analyzed via high-resolution MS without TiO₂ enrichment, and phosphorylation sites identified through peptide ionization patterns compared to the non-phosphorylated primary amino acid sequence.

Partial Protocol Preview
This section provides a glimpse into the protocol.
The remaining content is hidden due to licensing restrictions, but the full text is available at the following link: Access Free Full Text.

Schafer C.T., Chen Q., Tesmer J.J, & Handel T.M. (2023). Atypical Chemokine Receptor 3 ‘Senses’ CXC Chemokine Receptor 4 Activation Through GPCR Kinase Phosphorylation. bioRxiv.

Publication Preprint 2023

Ackr3 Amino acid sequence Grk2 Peptide Phosphorylation Trypsin

Corresponding Organization : Purdue University West Lafayette

Other organizations : Indiana University – Purdue University Indianapolis

Top 5 similar protocols

Variable analysis

independent variables

ACKR3 in LMNG
ACKR3 in nanodisc

dependent variables

Phosphorylation sites identified through peptide ionization patterns

control variables

Trypsin digestion
High-resolution MS without TiO2 enrichment

controls

Non-phosphorylated primary amino acid sequence (as a negative control)

Annotations

Based on most similar protocols

Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.

As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!