NaCl Effects on Bacterial Growth and Viability

The effect of NaCl on the growth of bacteria was studied on a Luria–Bertani (Thermo Fisher Scientific, Waltham, MA, USA) medium with the addition of various concentrations of NaCl (1%, 5%, 10%, 15%, and 20%) [47 (link)]. The Petri dishes were incubated at a temperature of 28 °C. The growth of bacteria was noted visually after 72 h. The effect of NaCl on the cell number of TS3 was studied in the liquid LB medium supplemented with various concentrations of NaCl (1%, 5%, 10%, 15%, and 20%). The TS3 was cultured for 3 days at 28 °C in 500 mL flasks placed in a rotary shaker (180 rpm). Then, aliquots (100 µL) of the serial dilutions (10⁻¹–10⁻⁵) were inoculated onto LB plates. The plates were maintained at 28 °C for 3 days, and the cell number was calculated.