ELISA Assay for IgG Reactivity
Corresponding Organization : University of Copenhagen
Other organizations : Florida Atlantic University, University of Ghana, Noguchi Memorial Institute for Medical Research, Kwame Nkrumah University of Science and Technology, Rigshospitalet
Variable analysis
- Recombinant proteins used to coat the 96-well plates
- IgG reactivity against recombinant proteins, measured by ELISA and calculated in arbitrary units
- Plasma samples (1:400 dilution)
- Horseradish peroxidase–conjugated rabbit anti-human IgG (1:3000 dilution)
- TMB PLUS2 for detection
- Sulfuric acid to stop the reaction
- Optical density read at 450 nm
- Negative control: Plasma samples from Danish adults without malaria exposure
- Positive control: Pool of Ghanaian adults with previous P. falciparum infection
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