Photocrosslinkable Hybrid Hydrogel Synthesis

The PVA-g-GMA (100 mmol of GMA) was dissolved in 10% v/v DMSO in deionized water to obtain a 10% (w/v) solution, stirred at 60 °C until a homogeneous solution was achieved. The 490 mM SF-g-GMA sponge was dissolved in deionized water at 50% w/v at room temperature. The mixed solutions of PVA-g-GMA and SF-g-GMA were thoroughly blended at different ratios of PVA-g-GMA to SF-g-GMA: 100/0, 75/25, 50/50, 25/75, and 0/100 (w/w of dry substances), followed by the addition of 0.3% (w/v) LAP. All gels were crosslinked with UV light (365 nm) for 10 min at an intensity of 6 mW/cm². Molds were utilized to create a 96-well plate with a well diameter of 6.72 mm.