MDA-MB-231 cells were cotransfected with pcDNA3.1-MS2, pcDNA3.1-MS2- NUDT3-AS4, pcDNA3.1-MS2- NUDT3-AS4 –Mut (miR-99s) and pMS2-GST. After 36 h, cells were used to perform RNA immunoprecipitation (RIP) experiments and RNA purification using GSH agarose beads (GE Healthcare) and the Magna RIP™ RNA-Binding Protein Immunoprecipitation Kit (Millipore, Bedford, MA) as described previously22 (link). pMS2-GST and pMS2 plasmid were gifts from Dr. Gorospe22 (link). The RNA fraction isolated by RIP was quantified by NanoDrop ND1000 (Thermo-Fisher Scientific, Waltham, MA) and its quality was assessed by Agilent 2100 Bioanalyzer (Agilent).
Hao Q., Wang P., Dutta P., Chung S., Li Q., Wang K., Li J., Cao W., Deng W., Geng Q., Schrode K., Shaheen M., Wu K., Zhu D., Chen Q.H., Chen G., Elshimali Y., Vadgama J, & Wu Y. (2020). Comp34 displays potent preclinical antitumor efficacy in triple-negative breast cancer via inhibition of NUDT3-AS4, a novel oncogenic long noncoding RNA. Cell Death & Disease, 11(12), 1052.
Other organizations :
Charles R. Drew University of Medicine and Science, Shanghai East Hospital, Guangdong Academy of Medical Sciences, Guangdong Provincial People's Hospital, Wuhan University, Renmin Hospital of Wuhan University, Stony Brook University, California State University, Fresno
Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.
As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to
get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
