Western Blot Analysis of Liver Proteins

The protein samples were extracted from the frozen livers according to previous protocols^{37 (link)}. The samples dissolved in the Yeasen Biotech sodium dodecyl sulphate (SDS)-loading buffer (Shanghai, China) were electrophoresed and transferred to Merck Millipore polyvinylidene difluoride (PVDF) membranes (Billerica, MA, USA) and then subjected to immunoblot analysis with the antibodies against p-JNK, JNK, p-cPLA2, cPLA2, platelet-type 12-LOX and leukocyte-type 12-LOX. The images were captured by the CLINX ChemiScope 3300 mini system (Shanghai, China). The relative protein abundance in each group was quantified by National Institute of Health Image J software (Silver Springs, MD, USA).