C57BL/6J wild-type male mice were obtained from Beijing Vital River Laboratory. GAD2-Cre mice (stock no. 028867, The Jackson Laboratory) and PV-Cre mice (stock no. 017320, The Jackson Laboratory) consisted of the GAD2-IRES-Cre and B6 PV-Cre knock-in lines, respectively. GAD67-GFP mice were a gift from N. Tamamaki (Kumamoto University) (61 (link)). The c-fos-CreERT2 mouse line (23 (link)) was obtained from Shanghai Model Organisms (stock no. NM-KI-200110) for TRAP experiments.
Mice were housed in a room maintained at a constant temperature on a 12-hour light/dark cycle (light from 08:00 to 20:00). All transgenic and knock-in mouse lines were maintained as hemizygotes. Mice had access to food and water ad libitum and were socially housed in numbers of two to five littermates randomly until surgery. Following surgery, mice were housed undisturbed. All mice were male and 3 to 5 months old for behavioral experiments. All procedures were approved by the Institutional Animal Care and Use Committee of the Fourth Military Medical University following Animal Research: Reporting of In Vivo Experiments (ARRIVE) guidelines (FMMULL-220926) and conformed to the Guide for the Care and Use of Laboratory Animals published by the National Institutes of Health. Animals were randomized to different treatments according to a group of pregenerated random numbers by Excel.
Mice were housed in a room maintained at a constant temperature on a 12-hour light/dark cycle (light from 08:00 to 20:00). All transgenic and knock-in mouse lines were maintained as hemizygotes. Mice had access to food and water ad libitum and were socially housed in numbers of two to five littermates randomly until surgery. Following surgery, mice were housed undisturbed. All mice were male and 3 to 5 months old for behavioral experiments. All procedures were approved by the Institutional Animal Care and Use Committee of the Fourth Military Medical University following Animal Research: Reporting of In Vivo Experiments (ARRIVE) guidelines (FMMULL-220926) and conformed to the Guide for the Care and Use of Laboratory Animals published by the National Institutes of Health. Animals were randomized to different treatments according to a group of pregenerated random numbers by Excel.
