Electrophysiological Recording of Cat Hindlimb Motor Units
All procedures described below were approved by The Institutional Animal Care and Use Committee at Temple University. As described previously (Zaback et al. 2022 (link)), an adult female cat was provided atropine (0.05 mg/kg IM) and anesthetized with isoflurane (1.5–3.5% in oxygen), during which a series of surgical procedures were performed including L3 laminectomy, implantation of nerve cuffs on the tibial and sural nerve, and isolation of hindlimb muscles. Individual Myomatrix threads were implanted in hindlimb muscles using the “intramuscular” method described above. Following these procedures, a precollicular decerebration was performed and isoflurane was discontinued. Following a recovery period, the activity of hindlimb motor units were recorded in response to electrical stimulation of either the contralateral tibial nerve or the ipsilateral sural nerve.
Chung B., Zia M., Thomas K.A., Michaels J.A., Jacob A., Pack A., Williams M.J., Nagapudi K., Teng L.H., Arrambide E., Ouellette L., Oey N., Gibbs R., Anschutz P., Lu J., Wu Y., Kashefi M., Oya T., Kersten R., Mosberger A.C., O’Connell S., Wang R., Marques H., Mendes A.R., Lenschow C., Kondakath G., Kim J.J., Olson W., Quinn K.N., Perkins P., Gatto G., Thanawalla A., Coltman S., Kim T., Smith T., Binder-Markey B., Zaback M., Thompson C.K., Giszter S., Person A., Goulding M., Azim E., Thakor N., O’Connor D., Trimmer B., Lima S.Q., Carey M.R., Pandarinath C., Costa R.M., Pruszynski J.A., Bakir M, & Sober S.J. (2023). Myomatrix arrays for high-definition muscle recording. bioRxiv.
Other organizations :
Georgia Institute of Technology, Western University, Columbia University, Champalimaud Foundation, Otto-von-Guericke University Magdeburg, Tufts University, Johns Hopkins University, Johns Hopkins Medicine, University Hospital Cologne, Salk Institute for Biological Studies, University of Colorado Anschutz Medical Campus, Drexel University, Temple University, Allen Institute
Electrical stimulation of the contralateral tibial nerve
Electrical stimulation of the ipsilateral sural nerve
dependent variables
Activity of hindlimb motor units
control variables
Adult female cat
Atropine administration (0.05 mg/kg IM)
Isoflurane anesthesia (1.5–3.5% in oxygen)
L3 laminectomy
Implantation of nerve cuffs on the tibial and sural nerves
Isolation of hindlimb muscles
Intramuscular implantation of Myomatrix threads in hindlimb muscles
Precollicular decerebration
Discontinuation of isoflurane after recovery period
positive controls
None specified
negative controls
None specified
Annotations
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