Nematode isolates of G. pallida, G. rostochiensis, G. tabacum, Heterodera sp. and different mixtures of G. pallida/G. rostochiensis (Table 1 ) were obtained at the Nematology lab of INIAV (NemaINIAV, Oeiras, Portugal).
The extraction of total DNA was always conducted using the Qiagen DNeasy Blood and Tissue kit (Life Technologies, Carlsbad, CA, USA), following the manufacturer’s instructions. Genomic DNA was quantified using the thermo-NANODROP 2000 spectrophotometer (Thermo Fisher Scientific, Waltham, MA, USA) and stored at −20 °C until further use. DNA extracts were used directly for the PCR reactions without any additional purification step. Total DNA extraction, purification and conservation was performed as described in Camacho et al. [18 (link)].
The extraction of total DNA was always conducted using the Qiagen DNeasy Blood and Tissue kit (Life Technologies, Carlsbad, CA, USA), following the manufacturer’s instructions. Genomic DNA was quantified using the thermo-NANODROP 2000 spectrophotometer (Thermo Fisher Scientific, Waltham, MA, USA) and stored at −20 °C until further use. DNA extracts were used directly for the PCR reactions without any additional purification step. Total DNA extraction, purification and conservation was performed as described in Camacho et al. [18 (link)].
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