Validating Optimized One-Step RT-RPA Method

The robustness of the optimized one-step RT-RPA method was further validated by testing 99 PLRV suspected field samples (leaves and tubers) of popular potato cultivars during the year 2019–2020. These samples were collected from fields across the states of India. The states of India that are believed to have contributed the suspect samples are as follows: Bihar, Gujarat, Haryana, Himachal Pradesh, Madhya Pradesh, Meghalaya, Punjab, Uttar Pradesh and West Bengal. Virus-free tissue culture-grown mother plants and mini-tubers were used as the samples for determining the overall health of the population. The validation procedure also made use of the tubers that had been saved from diseased plants. In addition to examining dormant tubers (without sprouts), sprouts and leaves, a comparison between DAS-ELISA and one-step RT-PCR/RT-RPA was conducted. Comparable results from this investigation were discovered (Table 2 and Table 3). The RT-PCR and DAS-ELISA procedures were carried out in the same way as previously described [12 (link)].

Free full text: Click here

Kumar R., Kaundal P., Tiwari R.K., Lal M.K., Kumari H., Kumar R., Naga K.C., Kumar A., Singh B., Sagar V, & Sharma S. (2023). Development of Reverse Transcription Recombinase Polymerase Amplification (RT-RPA): A Methodology for Quick Diagnosis of Potato Leafroll Viral Disease in Potato. International Journal of Molecular Sciences, 24(3), 2511.

Publication 2023

Elisa Mother Plants Population health Potato Rt pcr Tissue Tubers Virus plants

Corresponding Organization : Central Potato Research Institute

Other organizations : Central Rice Research Institute

Top 5 similar protocols

Variable analysis

independent variables

Testing of PLRV suspected field samples (leaves and tubers) of popular potato cultivars

dependent variables

PLRV detection in the samples
Comparison of results between DAS-ELISA and one-step RT-PCR/RT-RPA

control variables

Virus-free tissue culture-grown mother plants and mini-tubers used as samples for determining overall health of the population
Tubers saved from diseased plants

controls

Positive control: Virus-free tissue culture-grown mother plants and mini-tubers
Negative control: Not explicitly mentioned

Annotations

Based on most similar protocols

Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.

As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!