Optimized Liposomal Irinotecan Formulation

According to the BBD results, CPT-11-Lip and CA-CPT-11-Lip (n = 3) were prepared under the optimal process. Free-formed drug and liposomes were separated using a Sephadex G-50 gel chromatography method [29 (link)]. The inner diameter of the chromatographic column was 2.5 cm. The loading height of the dextran gel G-50 was about 15 cm. The sample volume of liposome was 1 mL, and pure water was used as the elution solvent with a flow rate of 0.5 mL/min. Formula (1) and (2) were used to calculate the EE and DL of liposomes, respectively.

EE % = \frac{encapsulated drug content}{total drug content} \times 100 %

DL % = \frac{encapsulated drug content}{weight of carrier} \times 100 %

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Zhou T., Liu Y., Lei K., Liu J., Hu M., Guo L., Guo Y, & Ye Q. (2023). A “Trojan Horse” Strategy: The Preparation of Bile Acid-Modifying Irinotecan Hydrochloride Nanoliposomes for Liver-Targeted Anticancer Drug Delivery System Study. Molecules, 28(4), 1577.

Publication 2023

Chromatographic Cpt 11 Dextran Drug Drug carrier Drug drug Gel chromatography Liposomes Sephadex g 50 Solvent

Corresponding Organization : Chengdu University of Traditional Chinese Medicine

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Variable analysis

independent variables

Preparation process (optimal process)

dependent variables

Encapsulation efficiency (EE%)
Drug loading (DL%)

control variables

Chromatographic column diameter (2.5 cm)
Dextran gel G-50 loading height (15 cm)
Liposome sample volume (1 mL)
Elution solvent (pure water)
Flow rate (0.5 mL/min)

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