Measurement of HDAC Activity in Amygdala and BNST

HDAC activity in the amygdala and BNST tissues was measured, as described by us previously (Pandey et al., 2008 (link); Sakharkar et al., 2012 (link); 2014 (link)). In brief, cytosolic and nuclear protein fractions were prepared using a nuclear extraction kit (Sigma) and HDAC activity (HDAC class I and II) was assayed using the colorimetric HDAC activity assay kit (BioVision Research, Mountain View, CA). The enzymatic activity was calculated as O.D. /mg protein and then represented as the mean ± SEM.

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Sakharkar A.J., Tang L., Zhang H., Chen Y., Grayson D.R, & Pandey S.C. (2014). Effects of Acute Ethanol Exposure on Anxiety Measures and Epigenetic Modifiers in the Extended Amygdala of Adolescent Rats. The international journal of neuropsychopharmacology / official scientific journal of the Collegium Internationale Neuropsychopharmacologicum (CINP), 17(12), 2057-2067.

Publication 2014

nuclear extraction kit

Amygdala Assay Colorimetric Cytosolic Enzymatic activity Nuclear protein Protein Tissues

Corresponding Organization :

Other organizations : University of Illinois at Chicago

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Variable analysis

independent variables

Tissue type (amygdala and BNST)

dependent variables

HDAC activity (HDAC class I and II)

control variables

Cytosolic and nuclear protein fractions prepared using a nuclear extraction kit (Sigma)
HDAC activity assayed using the colorimetric HDAC activity assay kit (BioVision Research, Mountain View, CA)

controls

Positive control: Not explicitly mentioned
Negative control: Not explicitly mentioned

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