The line profiles obtained from the SICM images and confocal images were analyzed with a FFT algorithm to retrieve regular organization or positioning of the T-tubules or Z-grooves. The power spectrum of the desired line profile was extracted from 2D topographic SICM images by the XEI analysis program (Park Systems). The line profiles and power spectrums of the confocal images were analyzed with the Zen 2011 program (Zeiss). From the retrieved power spectrum peaks, we determined the regularly repeating appearance of T-tubules or Z-grooves. The FFT analysis was used to evaluate the presence of T-tubules and its remodeling before and after the formamide-induced detubulation protocol [5 (link)].
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FFT Analysis of T-tubule Remodeling
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Corresponding Organization :
Other organizations : Samsung Medical Center, Sungkyunkwan University
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Variable analysis
independent variables
- Formamide-induced detubulation protocol
- Regular organization or positioning of the T-tubules or Z-grooves
- Presence of T-tubules and its remodeling
- Not explicitly mentioned
- Positive control: Not specified
- Negative control: Not specified
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