Immunolabeling of Retinal Cell Types

The following primary antibodies were used: anti-EGFP (chicken; Life Technologies, A10262; 1:500), rabbit anti-Rx2 (Reinhardt et al., 2015 (link); 1:500), anti-tagRFP (rabbit; Evrogen, AB233; 1:500), anti-Pax6 (rabbit; Hiss Diagnostics, PRB-278P; 1:200), PKCα (rabbit; Santa Cruz, sc-208; 1:200), anti-GS (mouse; Chemicon, MAB302; 1:500), anti-Sox2 (rabbit; Genetex, GTX101506; 1:500), anti-HuC/D (mouse; Thermo Fisher, A21271; 1:500), anti-recoverin (rabbit; Millipore, AB5585; 1:500), anti-Zpr-1 (mouse; Zebrafish International Resource Center; 1:500), anti-DsRed (rabbit; Clontech, 632496; 1:500), anti-BrdU (rat; AbD Serotec, BU1/75; 1:200). The following secondary antibodies were used: anti-mouse Cy5 (Jackson ImmunoResearch, 715-175-151), anti-chicken 488 (Jackson ImmunoResearch, 703-485-155), anti-rat DyLight549 (Jackson ImmunoResearch, 112-505-143), anti-rabbit DyLight549 (Jackson ImmunoResearch), anti-mouse Alexa546 (Life Technologies, A-11030) and anti-rat Alexa633 (Life Technologies, A21094). DAPI (Sigma-Aldrich, D9564) nuclear counterstaining was performed as described by Inoue and Wittbrodt (2011) (link).

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Pérez Saturnino A., Lust K, & Wittbrodt J. (2018). Notch signalling patterns retinal composition by regulating atoh7 during post-embryonic growth. Development (Cambridge, England), 145(21), dev169698.

Publication 2018

A10262 PRB-278P A21271 BU1/75 anti-mouse Cy5 anti-chicken 488 anti-rat DyLight549 anti-rabbit DyLight549 anti-mouse Alexa546 anti-rat Alexa633 DAPI

Antibodies Brdu Chicken Dapi Diagnostics Mouse Pkcα Rabbit Recoverin Sox2 Zebrafish

Corresponding Organization :

Other organizations : Heidelberg University

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Variable analysis

independent variables

None explicitly mentioned

dependent variables

The expression/localization of the following proteins: EGFP, Rx2, tagRFP, Pax6, PKCα, GS, Sox2, HuC/D, recoverin, Zpr-1, DsRed, BrdU

control variables

None explicitly mentioned

controls

Positive controls: None mentioned
Negative controls: None mentioned

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