Iron Metabolism in Cocaine Use Disorder

We recruited 44 individuals (95% male) with a chronic history of cocaine use, meeting the DSM-IV-TR criteria for cocaine dependence, and 44 matched healthy control volunteers (93% male) without a history of drug or alcohol dependence. The diagnosis of cocaine dependence was ascertained using the Structured Clinical Interview for DSM-IV, and these individuals are subsequently referred to as cocaine use disorder (CUD). None of the control participants had ever met DSM-IV-TR criteria for substance dependence; for further details see Supplementary Material. All participants provided written informed consent before they underwent a medical review and psychiatric screening. Exclusion criteria included major medical or neurological illness, lifetime history of a psychotic disorder, history of a traumatic head injury, or any contra-indications to MR-scanning. Dietary iron intake was calculated from the Food Frequency Questionnaire (http://www.srl.cam.ac.uk/epic/nutmethod/FFQ.shtml). Diet-related variations in iron absorption were estimated using the algorithms developed by Hallberg and Hulthen.^{33 (link)} All participants provided blood samples for the analysis of iron proteins in serum (that is, ferritin, iron, transferrin), hepcidin-25, acute inflammation (that is, C-reactive protein (CRP)) and haematological status. This study was approved by the National Research Ethics Committee (12/EE/0519; PI: KDE).

Free full text: Click here

Ersche K.D., Acosta-Cabronero J., Jones P.S., Ziauddeen H., van Swelm R.P., Laarakkers C.M., Raha-Chowdhury R, & Williams G.B. (2017). Disrupted iron regulation in the brain and periphery in cocaine addiction. Translational Psychiatry, 7(2), e1040-.

Publication 2017

Alcohol dependence Blood analysis Blood proteins C reactive protein Cocaine Cocaine dependence Diagnosis Diet iron Drug Ferritin Food Head traumatic injury Healthy volunteers Hepcidin Inflammation Iron Male Proteins Psychotic disorder Research ethics committee Serum Substance dependence Transferrin

Corresponding Organization :

Other organizations : University of Cambridge, German Center for Neurodegenerative Diseases, Wellcome/MRC Institute of Metabolic Science, Wellcome Trust, Radboud University Nijmegen, Radboud University Medical Center

Top 5 similar protocols

Protocol cited in 4 other protocols

VRK1 Depletion Across Cell Lines

The depletion of VRK1 by siRNA has been previously reported for A549 [20 (link), 23 (link)], H1299 [20 (link), 23 (link)], MDA-MB-231 [26 (link)] and HT144 [23 (link), 24 (link)] cell lines. Specific VRK1 knockdown was performed using siVRK1–02 from DHARMACON RNA Technologies. The target sequences of this siVRK1–02 is the following one (5′ to 3′): CAAGGAACCUGGUGUUGAA. The “ON-TARGETplus siCONTROL Non-targeting siRNA” from DHARMACON was used as negative control (siCtrl) [31 (link)]. Briefly, cells were transfected with the indicated siRNA at a concentration of 20 nM using either Lipofectamine 2000 (Invitrogen) or Lipotransfectin (Nivorlab), following manufacturer’s instructions [25 (link), 31 (link), 32 (link)].

Campillo-Marcos I, & Lazo P.A. (2019). Olaparib and ionizing radiation trigger a cooperative DNA-damage repair response that is impaired by depletion of the VRK1 chromatin kinase. Journal of Experimental & Clinical Cancer Research : CR, 38, 203.

+ Open protocol

VRK1 and Sox2 Knockdown in Cells

VRK1 knockdown was performed using three different siRNA for VRK1 (NM_003384): siVRK1-02 (siV1-02), siVRK1-03 (siV1-03) and siVRK1-09 (siV1-09) (Dharmacon RNA Technologies,). The sequences targeted by these VRK1 siRNA oligonucleotides were siVRK1-01: GAAAGAGAGTCCAGAAGTA; siVRK1-02: CAAGGAACCTGGTGTTGAA; si-VRK1-03: GGAAUGGAAAGUAGGAUUA; and siVRK1-09: AGGUGUACUUGGUAGAUUA. Sox2 was knockdown with the SOX2 Trilencer-27 human siRNA (Origene, Rockville, MD). As negative control the “ON-TARGETplus siCONTROL Non-targeting siRNA” (siCt) (Dharmacon) was used. The efficiency of RNAi transfection was determined with “siGLO RISC-free siRNA” (DHARMACON) labelled with a red fluorochrome. All of them have been previously used. Cells were transfected with the indicated siRNA (20 nM), using Lipofectamine 2000 (Invitrogen; Carlsbad, CA) as previously reported16 (link)17 (link)28 (link)38 (link)57 (link).

Moura D.S., Fernández I.F., Marín-Royo G., López-Sánchez I., Martín-Doncel E., Vega F.M, & Lazo P.A. (2016). Oncogenic Sox2 regulates and cooperates with VRK1 in cell cycle progression and differentiation. Scientific Reports, 6, 28532.

+ Open protocol

Variable analysis

independent variables

Cocaine use disorder (CUD) group
Healthy control group

dependent variables

Serum iron proteins (ferritin, iron, transferrin)
Serum hepcidin-25
Serum C-reactive protein (CRP)
Hematological status

control variables

Sex (95% male in CUD group, 93% male in control group)
Absence of major medical or neurological illness
Absence of lifetime history of a psychotic disorder
Absence of history of a traumatic head injury
Absence of any contraindications to MR-scanning
Dietary iron intake calculated from Food Frequency Questionnaire
Diet-related variations in iron absorption estimated using algorithms developed by Hallberg and Hulthen

Annotations

Based on most similar protocols

Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.

As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!