Quantitative Assessment of Plasma Lipid Profile and Oxidative Stress Markers

The plasma total triglyceride (TG) content was determined quantitatively by a colorimetric (at 570 nm) Triglyceride Assay kit (Cayman Chemical, Ann Arbor, MI) according to instructions. Plasma cholesterol levels were determined by a Cholesterol Fluorometric Assay kit (Cayman Chemical), and total cholesterol content for each sample was estimated on the basis of relative fluorescence (excitation 550 nm, emission 590) obtained from standards. Plasma 8-isoprostane levels at 18 weeks were determined by a Cayman Enzyme Immunoassay Kit as described [8 (link), 17 (link)–19 (link)]. RBC reduced (GSH) and oxidized (GSSG) glutathione levels were assessed enzymatically by the DTNB-GSSG reductase method [17 (link)–19 (link)]. Plasma 3-nitrotyrosine (3-NT) results, determined by the enzyme immunoassay components from Cayman Chemical, were derived from the concurrent study just published [8 (link)].

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ElZohary L., Weglicki W.B., Chmielinska J.J., Kramer J.H, & Mak I.T. (2019). Mg-supplementation attenuated lipogenic and oxidative/nitrosative gene expression caused by Combination Antiretroviral Therapy (cART) in HIV-1-transgenic rats. PLoS ONE, 14(1), e0210107.

Publication 2019

Triglyceride Assay kit Cholesterol Fluorometric Assay kit Enzyme Immunoassay Kit

8 isoprostane Assay Cayman Cholesterol Colorimetric Dtnb Enzyme immunoassay Fluorescence Fluorometric Glutathione Gssg Nitrotyrosine Plasma Reductase Triglyceride

Corresponding Organization : George Washington University

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Variable analysis

independent variables

Not explicitly mentioned

dependent variables

Plasma total triglyceride (TG) content
Plasma cholesterol levels
Plasma 8-isoprostane levels at 18 weeks
RBC reduced (GSH) and oxidized (GSSG) glutathione levels
Plasma 3-nitrotyrosine (3-NT) levels

control variables

Not explicitly mentioned

controls

Positive and negative controls not explicitly mentioned

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