Fungal Culture from Urine and HVS

The urine samples were centrifuged at 3000 rpm for 2 min and emulsified HVS samples in peptone water; 20 ul of the urine deposit or the emulsified HVS were spread on the SDA agar plate using a sterilized glass rod. Urine and the HVS samples were cultured on Sabourauds dextrose agar plates, containing 0.5 mg per 1000 ml chloramphenicol and incubated at 37 ^o C and examined for its growth at 24, 48 and 72 h. The culture plates were examined for the appearance, size, cream-coloured pastry colonies and morphology of the colonies [19 ].

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Asare K.K., Bentil H.A., Gyesi E., Amoah S., Bentsi-Enchill F, & Opoku Y.K. (2023). Candidiasis profile at the outpatient department of the university of cape coast hospital in the central region of Ghana: a retrospective study. BMC Women's Health, 23, 101.

Publication 2023

Agar Chloramphenicol Dextrose Peptone Urine

Corresponding Organization : University of Cape Coast

Other organizations : University of Education, Winneba

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Variable analysis

independent variables

Centrifugation at 3000 rpm for 2 min
Emulsification of HVS samples in peptone water
Spreading of 20 ul of urine deposit or emulsified HVS on SDA agar plate
Incubation of urine and HVS samples on SDA agar plates at 37 °C

dependent variables

Growth of colonies on SDA agar plates
Appearance, size, and morphology of colonies

control variables

SDA agar plates containing 0.5 mg per 1000 ml chloramphenicol
Examination of growth at 24, 48, and 72 hours

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