Western blotting was performed according to a previously described protocol4 (link). The following primary antibodies were used: anti-LAMP3 and anti-LAMP1 polyclonal antibodies (both purchased from Proteintech), and anti-FLAG, anti-α-tubulin, and anti-β-actin monoclonal antibodies (all three purchased from Sigma-Aldrich, St. Louis, MO, USA). The following secondary antibodies were used: Mouse IgG HRP-Linked Whole Antibody (GENA931) and Rabbit IgG HRP-Linked Whole Antibody (GENA934) (both purchased from Sigma-Aldrich). To confirm whether proteins were present, membranes were stained by using Reversible Protein Stain Kit (Thermo Fisher Scientific).
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