Antibody Characterization and Cell Assays

Rabbit anti-ATP1A1 mAb (catalog no. GY5154) was purchased from AbWays (Shanghai, China). CoraLite488-conjugated Goat Anti-Mouse IgG (catalog no. SA00013-1), CoraLite488-conjugated Goat Anti-Rabbit IgG (catalog no. SA00013-2), CoraLite594-conjugated Goat Anti-Mouse IgG (catalog no. SA00013-3), CoraLite594-conjugated Goat Anti-Rabbit IgG (catalog no. SA00013-4) and Mouse anti-GAPDH mAb (catalog no. 60004-1-Ig) were purchased from Proteintech (Wuhan, China). Mouse anti-PEDV-N mAb (catalog no. M100048) was purchased from Zoonogen (Beijing, China). Mouse anti-FLAG mAb (catalog no. F1804-200UG) was purchased from Sigma (St. Louis, MI, USA). Goat Anti-Rabbit IgG (catalog no. ab6721) and Goat Anti-Mouse IgG (catalog no. ab205719) were purchased from Abcam (Cambridge, UK). The PEDV S1 mAb was a generous gift from Professor Kegong Tian of Henan Agricultural University, China. In Western blot experiments, the primary antibody was diluted 1:1000 and the secondary antibody was diluted 1:5000. In the IFA assay, the antibody was diluted 1:300.
Ouabain (catalog no. HY-B0542) and PST2238 (catalog no. HY-12283) were purchased from MCE (Shanghai, China).
Lipofectamine RNAiMAX transfection reagent (catalog no. 13778150) was purchased from Invitrogen. FuGENE transfection reagent was purchased from Promega (Madison, WI, USA). Cell lysis buffer for Western and IP (catalog no. P0013, Beyotime, China) was purchased from Beyotime (Shanghai, China). Cell Counting Kit-8 was purchased from YEASEN (Shanghai, China).